Real-time PCR or quantitative PCR (Polymerase Chain Reaction) is an advancement of the standard PCR technique, which enables continuous monitoring of the reaction and collection of data in real time.

Multiplex PCR is a difficult technique that allows amplification of two or more products in parallel in a single reaction tube. This technology was first described by Chamberlain et al., in 1988, and ...

Digital PCR, or dPCR, is a molecular biology technique that detects and quantifies nucleic acid targets. By partitioning a sample into many individual reactions, scientists can measure whether a ...

The alternative text for this image may have been generated using AI. At this time, there are several commercial sequencing platforms available, each with advantages and disadvantages. The platforms ...

In the six decades since Watson and Crick identified the double helical structure of DNA, a series of elegant and powerful experiments has established the broad outlines of how genetic information is ...

First described in the 1990s, digital PCR is a novel approach to PCR which allows precise detection and quantification of the amount of nucleic acids formed during PCR. It is different from ...

PCR duplicates—sequencing reads from the same original genomic fragment—can cause headaches. But there are remedies. You have full access to this article via your institution. Plenty can go wrong when ...